Provided herein are methods, compositions and systems for analyzing individual cells or a small population of cells, including the analysis and attribution of the analytes from and to these individual cells or cell populations.ĭisclosed herein, in some embodiments, is a method for processing a major histocompatibility complex (MHC) molecule, comprising: (a) providing a droplet or well comprising (i) the MHC molecule and (ii) a particle having at least one peptide molecule and at least one nucleic acid molecule comprising a peptide barcode sequence coupled thereto (b) attaching the at least one peptide molecule and the at least one nucleic acid molecule to the MHC molecule, to yield a derivative of the WIC molecule and (c) recovering the derivative of the WIC molecule from the droplet or well. Such cells include, but are not limited to, cancer cells, fetal cells, and immune cells involved in immune responses. Recognized herein is the need for methods, compositions, and systems for analyzing multiple analytes (e.g., genomic, epigenomic, transcriptome, and/or proteomic information) from individual cells or a small population of cells. Samples in partitions may be subjected to heating or cooling, or chemical reactions, such as to yield species that may be qualitatively or quantitatively processed. For example, such droplets may be fluidically isolated from other droplets, enabling accurate control of respective environments in the droplets.īiological samples in partitions may be subjected to various processes, such as chemical processes or physical processes. Droplets or wells may be employed to process biological samples in a manner that enables the biological samples to be partitioned and processed separately.
There are various approaches for processing samples, such as polymerase chain reaction (PCR) and sequencing.īiological samples may be processed within various reaction environments, such as partitions. Biological samples may be processed, such as for detection of a disease (e.g., cancer) or identification of a particular species. BACKGROUNDĪ sample may be processed for various purposes, such as detection, identification, quantitation, and characterization of a type of moiety within the sample. The instant application contains a Sequence Listing which has been filed electronically in ASCII format and is hereby incorporated by reference in its entirety. 12, 2019, which claims the benefit of U.S. This application is a continuation of International Application No.
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